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ResultsLIMNOCARDIIDAE (MOLLUSCA:BIVALVIA) PONTO-CASPIAN RELICTS IN ROMANIAN FAUNA - TAXONOMICAL STATUS, FAUNISTICS, BIOLOGY AND POPULATION GENETIC STRUCTURE
RESULTS 2007
It is the first systematic study of the Limnocardiidae family in Romania in aproximately 50 years. The collected material was identified and introduced in the scientific collections of the „Grigore Antipa” National Museum of Natural History, thus, making possible to use the material from the museum collections for taxonomic studies which are based on molecular biology techniques.
- Organising the MNINGA Limnocardiidae collection
RESULTS 2008
- Organisation of a DNA collection with
genetic material from all Limnocardiidae species found in
DNA was extracted from individuals collected
during this project as well as from individuals preserved in the collections of
the „Grigore Antipa” National Museum of Natural History. This DNA material is
organised in new collection, of great importance , being unique in Europe. DNA
was isolated from 314 individuals belonging to the four Limnocardiidae species
found in
Published papers
-Oana P. Popa, Andrei Sarkany-Kiss, Beatrice S. Kelemen, Elena I. Iorgu Dumitru Murariu, Luis O. Popa, 2009 , Contributions to the knowledge of the presemt Limnocardiidae fauna (Mollusca: Bivalvia) from Romania. Travaux du Museum D`Histoire Naturelle Grigore Antipa, LII: 7-15. www.travaux.ro
RESULTS 2009
-Published papers
-Oana P. Popa, Elena I. Iorgu , Beatrice S. Kelemen, Andrei Sarkany-Kiss, Dumitru Murariu, Luis O. Popa, Morphometric analysis in some populations of Limnocardiids species from Lake Razelm (Romania), Travaux du Museum D`Histoire Naturelle Grigore Antipa, in press.
- Participation in national / international scientific conferences
-Oana Paula Popa, Elena Iulia Iorgu, Beatrice Simona Kelemen, Andrei Sarkany-Kiss, Dumitru Murariu, Luis Ovidiu Popa, Morphometric analysis in some populations of Limnocardiids species from Razelm Lake (Romania), Annual Zoological Congress of Grigore Antipa Museum, 12-13 November 2009, Bucharest, Romania, oral presentation.
-Luis Ovidiu Popa, Oana Paula Popa, Elena Iulia Iorgu, Lack of divergence between 16S and 18S DNA sequences of Monodacna pontica and M. colorata (Bivalvia: Cardiidae) from Goloviţa Lake, , Annual Zoological Congress of Grigore Antipa Museum, 12-13 November 2009, Bucharest, Romania, oral presentation.
- We have submitted to public databases, several DNA sequences of three amplified molecular markers used in phylogenetic analysis (ITS2, 16S and 18s RNAr genes) from each putative Limnocardiidae species
RESULTS 2010 Published papers:
1. L. Popa, O. Popa, E. Iorgu, B. Kelemen, D. Murariu - Molecular insights into the taxonomy of Hypanis (Bivalvia: Cardiidae: Lymnocardiinae) in the Black Sea lagoons. Revista: Helgoland Marine Research (under revision). Impact factor: 1,115
2. Popa,O.P., Iorgu,E.I., Krapal,A.-M., Kelemen,B.S., Murariu,D. Popa,L.O. - Isolation and characterization of the first microsatellite markers for the endangered relict mussel Hypanis colorata (Mollusca: Bivalvia: Cardiidae). International Journal of Molecular Sciences. (under revision). Impact factor: 1.387
Collaboration
with foreign institutions Dr. Sci. Vitaliy V. Anistratenko -
Head of the Lab of Zoogeography Shmalhausen Institute of Zoology NAS of the
Ukraine. Prof dr. R.G. Moolenbeeck. - Curator of the
Malacological Collections, Zoological Museum of Amsterdam, University of
Amsterdam, Netherlands. Frank P. Wesselingh - Curator of the
Malacological Collections, „Naturalis” National Museum of Natural History
Leyden, Netherlands. Jan Johan ter Poorten – owner of the most importnat private collection of Cardiidae from Europe. BEATRICE SIMONA KELEMEN PhD Activities performed in the project: - Documentation on Limnocardiidae species from Romanian fauna. - Sampling, sorting and identification of biological material. - DNA extraction - Optimisation of protocols for PCR amplification of target genes from Limnocardiidae species. - Sequencing the target genes (16S, 18S, ITS2) and analysis of the results. - Participation in the elaboration of scientific papers published from the project - Research visits at the Zoological Museum of the University of Amsterdam and Naturalis National Museum of Natural History in Leiden - Malacological Department (7-17 october 2008) OANA PAULA POPA PhD Activities performed in the project:: - Documentation on Limnocardiidae species from Romanian fauna - Sampling, sorting and identification of biological material. - Organising a collection of Limnocardiidae in the Grigore Antipa National Museum of Natural History - Recording of the physio-chemical parametres from the studied habitats. - DNA extraction - Organising a Limnocardiidae DNA collection from the collected individuals. - Participation in the elaboration of scientific papers published from the project - Research visit at the Great Lake Institute for Environmental Research, Windsor University (1.04-2009-20.05.2009), trainning on DNA sequencing, primer design and phylogenetic analysis techinques. ELENA IULIA IORGU PhD fellow Activities performed in the project:: - Sampling and sorting of biological material. - Recording of the physio-chemical parametres from the studied habitats. - DNA extraction - Sequencing of the target genes. - Participation in the elaboration of scientific papers published from the project - Research visit at the Great Lake Institute for Environmental Research, Windsor University (1.04-2009-20.05.2009), trainning on DNA sequencing, primer design and phylogenetic analysis techinques.
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