Home | Objectives | Results | Photo Gallery | ro
Project team

Project Manager:

DR. Dumitru MURARIU

dmurariu@antipa.ro

Team Members:

DR. Luis Ovidiu POPA

popaluis@antipa.ro

DR. Oana Paula POPA

oppopa@antipa.ro

DR. Beatrice KELEMEN

bea_kelemen@yahoo.com

DR. Elena Iulia IORGU

elenap@antipa.ro

Results

LIMNOCARDIIDAE (MOLLUSCA:BIVALVIA) PONTO-CASPIAN RELICTS IN ROMANIAN FAUNA - TAXONOMICAL STATUS, FAUNISTICS, BIOLOGY AND POPULATION GENETIC STRUCTURE

RESULTS 2007

It is the first systematic study of the Limnocardiidae family in Romania in aproximately 50 years. The collected material was identified and introduced in the scientific collections of the „Grigore Antipa” National Museum of Natural History, thus, making possible to use the material from the museum collections for taxonomic studies which are based on molecular biology techniques.

- Organising the MNINGA Limnocardiidae collection

Taxon

Collecting point

Collecting date

Legit

No. of collected individuals

Monodacna genus


Monodacna colorata Eichwald 1829

Goloviţa Lake

2007, 2009

SBM

61

Razelm Lake

2009

SBM

302

Monodaca pontica Eichwald 1838

Goloviţa Lake

2007, 2008

SBM

68

Crapina Lake

1966

A. Negrea

35

Razelm Lake

1974

D. Manoleli

23

Razelm Lake

2009

SBM

188

Adacna genus


Adacna plicata relicta Milaschevich 1916

Goloviţa Lake

2007

SBM

1

Razelm Lake

1968

A. Grossu

3

Razelm Lake

1974

D. Manoleli

8

Razelm Lake

2009

SBM

54

Adacna fragilis Milaschevich 1908

Goloviţa Lake

2007, 2008

SBM

2

Razelm Lake

2009

SBM

30

TOTAL




775

RESULTS 2008

- Organisation of a DNA collection with genetic material from all Limnocardiidae species found in Romania.

DNA was extracted from individuals collected during this project as well as from individuals preserved in the collections of the „Grigore Antipa” National Museum of Natural History. This DNA material is organised in new collection, of great importance , being unique in Europe.

DNA was isolated from 314 individuals belonging to the four Limnocardiidae species found in Romania.

Published papers

-Oana P. Popa, Andrei Sarkany-Kiss, Beatrice S. Kelemen, Elena I. Iorgu Dumitru Murariu, Luis O. Popa, 2009 , Contributions to the knowledge of the presemt Limnocardiidae fauna (Mollusca: Bivalvia) from Romania. Travaux du Museum D`Histoire Naturelle Grigore Antipa, LII: 7-15. www.travaux.ro

RESULTS 2009

-Published papers

-Oana P. Popa, Elena I. Iorgu , Beatrice S. Kelemen, Andrei Sarkany-Kiss, Dumitru Murariu, Luis O. Popa,   Morphometric analysis in some populations of Limnocardiids species from Lake Razelm (Romania), Travaux du Museum D`Histoire Naturelle Grigore Antipa, in press.

- Participation in national / international scientific conferences

-Oana Paula Popa, Elena Iulia Iorgu, Beatrice Simona Kelemen, Andrei Sarkany-Kiss, Dumitru Murariu, Luis Ovidiu Popa, Morphometric analysis in some populations of Limnocardiids species from Razelm Lake (Romania), Annual Zoological Congress of Grigore Antipa Museum, 12-13 November 2009, Bucharest, Romania, oral presentation.

-Luis Ovidiu Popa, Oana Paula Popa, Elena Iulia Iorgu,  Lack of divergence between 16S and 18S DNA sequences of Monodacna pontica and M. colorata (Bivalvia: Cardiidae) from Goloviţa Lake, , Annual Zoological Congress of Grigore Antipa Museum, 12-13 November 2009, Bucharest, Romania, oral presentation.

- We have submitted to public databases, several DNA sequences of three amplified molecular markers used in phylogenetic analysis (ITS2, 16S and 18s RNAr genes) from each putative Limnocardiidae species

RESULTS 2010

Published papers:

1. L. Popa, O. Popa, E. Iorgu, B. Kelemen, D. Murariu - Molecular insights into the taxonomy of Hypanis (Bivalvia: Cardiidae: Lymnocardiinae) in the Black Sea lagoons. Revista: Helgoland Marine Research (under revision). Impact factor: 1,115

2. Popa,O.P., Iorgu,E.I., Krapal,A.-M., Kelemen,B.S., Murariu,D. Popa,L.O. - Isolation and characterization of the first microsatellite markers for the endangered relict mussel Hypanis colorata (Mollusca: Bivalvia: Cardiidae). International Journal of Molecular Sciences. (under revision). Impact factor: 1.387


Collaboration with foreign institutions

Prof. Melania Cristescu. Windsor University, Great Lake Institute for Environmental Research Ontario, Canada.

Dr. Sci. Vitaliy V. Anistratenko - Head of the Lab of Zoogeography Shmalhausen Institute of Zoology NAS of the Ukraine.

Prof dr. R.G. Moolenbeeck. - Curator of the Malacological Collections, Zoological Museum of Amsterdam, University of Amsterdam, Netherlands.

Frank P. Wesselingh - Curator of the Malacological Collections, „Naturalis” National Museum of Natural History Leyden, Netherlands.

Jan Johan ter Poorten – owner of the most importnat private collection of Cardiidae from Europe. 



The involvement of young researchers.

BEATRICE SIMONA KELEMEN PhD

Activities performed in the project:

-    Documentation on Limnocardiidae species from Romanian fauna.
-    Sampling, sorting and identification of biological material.
-    DNA extraction
-    Optimisation of protocols for PCR amplification of target genes from Limnocardiidae species.
-    Sequencing the target genes (16S, 18S, ITS2) and analysis of the results.
-    Participation in the elaboration of scientific papers published from the project
-    Research visits at the Zoological  Museum of the University of Amsterdam and Naturalis National Museum of Natural History in Leiden - Malacological Department (7-17 october 2008)

OANA PAULA POPA PhD

Activities performed in the project::

-    Documentation on Limnocardiidae species from Romanian fauna
-    Sampling, sorting and identification of biological material.
-    Organising a collection of Limnocardiidae in the Grigore Antipa National Museum of Natural History
-    Recording of the physio-chemical parametres from the studied habitats.
-    DNA extraction
-    Organising a Limnocardiidae DNA collection from the collected individuals.
-    Participation in the elaboration of scientific papers published from the project
-    Research visit at the Great Lake Institute for Environmental Research, Windsor University (1.04-2009-20.05.2009), trainning on DNA sequencing, primer design and phylogenetic analysis techinques.

ELENA IULIA IORGU PhD fellow

Activities performed in the project::

-    Sampling and sorting of biological material.
-    Recording of the physio-chemical parametres from the studied habitats.
-    DNA extraction
-    Sequencing of the target genes.
-    Participation in the elaboration of scientific papers published from the project
-    Research visit at the Great Lake Institute for Environmental Research, Windsor University (1.04-2009-20.05.2009), trainning on DNA sequencing, primer design and phylogenetic analysis techinques.

Personnel costs of young reserachers during 2007 - 2009

2007

60% of personnel costs

2008

60% of personnel costs

2009

60% of personnel costs



© www.cercetare-antipa.ro 2009