LIMNOCARDIIDAE (MOLLUSCA:BIVALVIA) PONTO-CASPIAN
RELICTS IN ROMANIAN FAUNA - TAXONOMICAL STATUS, FAUNISTICS, BIOLOGY AND
POPULATION GENETIC STRUCTURE
RESULTS
2007
It is the first systematic study of the
Limnocardiidae family in Romania in aproximately 50 years. The collected
material was identified and introduced in the scientific collections of the „Grigore
Antipa” National Museum of Natural History, thus, making possible to use the
material from the museum collections for taxonomic studies which are based on
molecular biology techniques.
- Organising the MNINGA Limnocardiidae
collection
Taxon
Collecting
point
Collecting
date
Legit
No.
of collected individuals
Monodacna
genus
Monodacna
colorataEichwald 1829
Goloviţa Lake
2007,
2009
SBM
61
Razelm
Lake
2009
SBM
302
Monodaca
ponticaEichwald 1838
Goloviţa Lake
2007,
2008
SBM
68
Crapina
Lake
1966
A.
Negrea
35
Razelm
Lake
1974
D.
Manoleli
23
Razelm
Lake
2009
SBM
188
Adacna
genus
Adacna
plicata relictaMilaschevich 1916
Goloviţa Lake
2007
SBM
1
Razelm Lake
1968
A.
Grossu
3
Razelm Lake
1974
D.
Manoleli
8
Razelm Lake
2009
SBM
54
Adacna
fragilis Milaschevich 1908
Goloviţa Lake
2007,
2008
SBM
2
Razelm
Lake
2009
SBM
30
TOTAL
775
RESULTS
2008
- Organisation of a DNA collection with
genetic material from all Limnocardiidae species found inRomania.
DNA was extracted from individuals collected
during this project as well as from individuals preserved in the collections of
the „Grigore Antipa” National Museum of Natural History. This DNA material is
organised in new collection, of great importance , being unique in Europe.
DNA
was isolated from 314 individuals belonging to the four Limnocardiidae species
found in Romania.
-Oana P. Popa, Elena
I. Iorgu , Beatrice S. Kelemen, Andrei Sarkany-Kiss, Dumitru Murariu, Luis O.
Popa, Morphometric analysis in some populations of
Limnocardiids species from Lake Razelm (Romania), Travaux du Museum
D`Histoire Naturelle Grigore Antipa, in press.
-
Participation in national / international scientific conferences
- We have submitted to public databases, several DNA sequences ofthree amplified molecular markers used in
phylogenetic analysis (ITS2, 16S and
18s RNAr genes) from each putative Limnocardiidae species
RESULTS 2010
Published papers:
1. L. Popa, O. Popa, E. Iorgu, B. Kelemen, D. Murariu - Molecular insights
into the taxonomy of Hypanis (Bivalvia: Cardiidae: Lymnocardiinae) in the Black
Sea lagoons. Revista: Helgoland Marine Research (under revision).Impact factor: 1,115
2. Popa,O.P., Iorgu,E.I., Krapal,A.-M.,
Kelemen,B.S., Murariu,D. Popa,L.O. - Isolation and characterization of the
first microsatellite markers for the endangered relict mussel Hypanis colorata
(Mollusca: Bivalvia: Cardiidae). International Journal of Molecular Sciences.
(under revision). Impact factor: 1.387
Collaboration
with foreign institutions
Prof. Melania Cristescu. Windsor
University, Great Lake Institute for Environmental Research Ontario, Canada.
Dr. Sci.Vitaliy V. Anistratenko -
Head of the Lab of Zoogeography Shmalhausen Institute of Zoology NAS of the
Ukraine.
Prof dr. R.G. Moolenbeeck. - Curator of the
Malacological Collections, Zoological Museum of Amsterdam, University of
Amsterdam, Netherlands.
Frank P. Wesselingh - Curator of the
Malacological Collections, „Naturalis” National Museum of Natural History
Leyden, Netherlands.
Jan Johan ter Poorten – owner of the most importnat private collection of Cardiidae from
Europe.
The involvement of young researchers.
BEATRICE SIMONA KELEMEN PhD
Activities performed in the project:
- Documentation on Limnocardiidae species from Romanian fauna. - Sampling, sorting and identification of biological material. - DNA extraction - Optimisation of protocols for PCR amplification of target genes from Limnocardiidae species. - Sequencing the target genes (16S, 18S, ITS2) and analysis of the results. - Participation in the elaboration of scientific papers published from the project - Research visits at the Zoological Museum of the University of Amsterdam and Naturalis National Museum of Natural History in Leiden - Malacological Department (7-17 october 2008)
OANA PAULA POPA PhD
Activities performed in the project::
- Documentation on Limnocardiidae species from Romanian fauna - Sampling, sorting and identification of biological material. - Organising a collection of Limnocardiidae in the Grigore Antipa National Museum of Natural History - Recording of the physio-chemical parametres from the studied habitats. - DNA extraction - Organising a Limnocardiidae DNA collection from the collected individuals. - Participation in the elaboration of scientific papers published from the project - Research visit at the Great Lake Institute for Environmental Research, Windsor University (1.04-2009-20.05.2009), trainning on DNA sequencing, primer design and phylogenetic analysis techinques.
ELENA IULIA IORGU PhD fellow
Activities performed in the project::
- Sampling and sorting of biological material. - Recording of the physio-chemical parametres from the studied habitats. - DNA extraction - Sequencing of the target genes. - Participation in the elaboration of scientific papers published from the project - Research visit at the Great Lake Institute for Environmental Research, Windsor University (1.04-2009-20.05.2009), trainning on DNA sequencing, primer design and phylogenetic analysis techinques.
Personnel costs of young
reserachers during 2007 - 2009