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Project team

Project Manager:

DR. Dumitru MURARIU

dmurariu@antipa.ro

Team Members:

DR. Luis Ovidiu POPA

popaluis@antipa.ro

DR. Oana Paula POPA

oppopa@antipa.ro

DR. Beatrice KELEMEN

bea_kelemen@yahoo.com

DR. Elena Iulia IORGU

elenap@antipa.ro

Objectives

Stage 1 - 2007. The 2007 objective was fully accomplished.

Objective 1. Investigation of Razelm-Sinoe lagoonal complex for collecting the Limnocardiidae species

1.1. Identification of the collecting sites based on literature and collecting samples.

1.2. Taxonomic identification of specimens from biological samples.

1..3. The analysis of the ecological and physico-chemical factors from Razelm-Sinoe Complex

1.4. Reporting the results to the financing body and filling the www.cercetatori-romani.ro site form.

Stage 2 - 2008. The 2008 objectives were fully accomplished.

Objective 2 . Present distribution of the Limnocardiidae species in Brates Lake, Crapina-Jijila pool complex

2.1. Identification of the collecting sites based on literature and collecting samples.

2.2. Taxonomic identification of specimens from biological samples

2.3. The analysis of the ecological and physico-chemical factors from Brates lake angd Crapina-Jijila ponds.

2.4. Analysis of distributional data.

2.5. Data analysis and interpretation of limnocardiidae ecological data.

Objective 3. Organising a Limnocardiidae DNA collection with species from Romanian fauna

3.1. Optimisation of DNA isolation protocols.

3.2. DNA isolation from all collected individuals.

3.3. Construction of a database based on the DNA collection

3.4. Reporting the results to the financing body and filling the www.cercetatori-romani.ro site form.

3.5. Participation in national / international scientific conferences and writing scientific papers.

Stage 3 - 2009. The 2009 objectives were fully accomplished.

Objective 4. Identifying the appropiate taxonomical status of the Romanian Limnocardiidae mussels.

4.1. Analysis of the mitochondrial CO1 (cytochrome oxidase I)and nuclear ITS2 (internal transcribed spacer) gene sequences from Limnocardiidae species.

4.2. Submittting the obtained sequences to the NCBI database.

4.3. Phylogenetic analysis of the Limnocardiidae family.

4.4. Participation in national / international scientific conferences and writing scientific papers.

Stage 4 - 2010

Objective 5. Identification of the genetic structure of Limnocardiidae populations present in the Romanian fauna

5.1. Microsatellite DNA variation analysis in studied populations.

5.2. Analysis of the RNA 16s gene sequences.

5.3. Publicarea secventelor obtinute in baza de date publica NCBI

5.4. Reporting the results to the financing body and filling the www.cercetatori-romani.ro site form.

5.5. Participation in national / international scientific conferences and writing scientific papers


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